pFlag-CMV2-mAIM2
(Plasmid
#51540)
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PurposeExpress Flag-tagged mAIM2 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51540 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFlag-CMV-2
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Backbone manufacturerSigma
- Backbone size w/o insert (bp) 4679
- Total vector size (bp) 5702
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAim2
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SpeciesM. musculus (mouse)
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Insert Size (bp)1062
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Entrez GeneAim2 (a.k.a. Gm1313, Ifi210)
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site SalI (destroyed during cloning)
- 5′ sequencing primer 5' pCMV2 primer
- 3′ sequencing primer 3' pCMV2 primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFlag-CMV2-mAIM2 was a gift from Emad Alnemri (Addgene plasmid # 51540 ; http://n2t.net/addgene:51540 ; RRID:Addgene_51540) -
For your References section:
AIM2 activates the inflammasome and cell death in response to cytoplasmic DNA. Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES. Nature. 2009 Mar 26;458(7237):509-13. doi: 10.1038/nature07710. Epub 2009 Jan 21. 10.1038/nature07710 PubMed 19158676
Map uploaded by the depositor.
