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pcDNA3-hAIM2deltPYD-T7
(Plasmid #51539)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51539 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Total vector size (bp) 6156
  • Modifications to backbone
    T7 tag, T7 promoter
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AIM2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    732
  • Mutation
    deletion of pyrin domain
  • Entrez Gene
    AIM2 (a.k.a. PYHIN4)
  • Promoter CMV
  • Tag / Fusion Protein
    • T7 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-hAIM2deltPYD-T7 was a gift from Emad Alnemri (Addgene plasmid # 51539 ; http://n2t.net/addgene:51539 ; RRID:Addgene_51539)

  • For your References section:

    AIM2 activates the inflammasome and cell death in response to cytoplasmic DNA. Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES. Nature. 2009 Mar 26;458(7237):509-13. doi: 10.1038/nature07710. Epub 2009 Jan 21. 10.1038/nature07710 PubMed 19158676
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