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Addgene

pET21a-hAIM2-PYD-GST
(Plasmid #51531)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51531 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5443
  • Total vector size (bp) 6390
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AIM2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    300
  • Mutation
    deletion of amino acids 102-343
  • Entrez Gene
    AIM2 (a.k.a. PYHIN4)
  • Promoter lac operator
  • Tags / Fusion Proteins
    • T7 (N terminal on backbone)
    • GST (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer His6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For protein expression the plasmid needs to be transformed into BL21 E. coli.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a-hAIM2-PYD-GST was a gift from Emad Alnemri (Addgene plasmid # 51531 ; http://n2t.net/addgene:51531 ; RRID:Addgene_51531)

  • For your References section:

    AIM2 activates the inflammasome and cell death in response to cytoplasmic DNA. Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES. Nature. 2009 Mar 26;458(7237):509-13. doi: 10.1038/nature07710. Epub 2009 Jan 21. 10.1038/nature07710 PubMed 19158676
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