DDC_M_Tub_KDEL
(Plasmid #51529)

• Purpose: Ectopic expression of DDC with KDEL tag for CTAP
• Depositing Lab: Claus Jorgensen
• Publication: Tape et al Mol Cell Proteomics. 2014 May 12.

Backbone

• Vector backbone: pCDNA3.1 (+) Zeo
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 6500
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DDC
• Alt name: Diaminopimelate Decarboxylase
• Species: Mycobacterium tuberculosis
• Insert Size (bp): 1500
• Promoter: CMV
• Tags / Fusion Proteins:
  • HA (C terminal on insert)
  • KDEL (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CGACTCACTATAGGGAGACCCAAGC
• 3′ sequencing primer: CCTTCCAGGGTCAAGGAAGGCACG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GeneArt
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry