pET21a-hAIM2-deltaPYD
(Plasmid #51522)

• Purpose: Express His6-tagged HIN domain of hAIM2 in bacteria
• Depositing Lab: Emad Alnemri
• Publication: Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. doi: 10.1038/nature07710. Epub 2009 Jan 21.

Backbone

• Vector backbone: pET21a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5443
• Total vector size (bp): 6141
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: AIM2
• Species: H. sapiens (human)
• Insert Size (bp): 732
• Mutation: deletion of pyrin domain
• Entrez Gene: AIM2 (a.k.a. PYHIN4)
• Promoter: lac operator
• Tags / Fusion Proteins:
  • T7 (N terminal on insert)
  • His6 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: His6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For protein expression the plasmid needs to be transformed into BL21 E. coli.