AAV pCAG-FLEX-tdTomato-WPRE
(Plasmid #51503)

• Purpose: Can be used to generate AAV virus that will express tdTomato in the presence of Cre
• Depositing Lab: Hongkui Zeng
• Publication: Oh et al Nature. 2014 Apr 10;508(7495):207-14. doi: 10.1038/nature13186. Epub 2014 Apr 2.

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Backbone

• Vector backbone: pAAV-MCS
• Backbone manufacturer: Stratagene
• Total vector size (bp): 7227
• Modifications to backbone: A fragment containing the pCAG promoter-FLEX-tdTomato-WPRE was swapped in to replace the CMV promoter-MCS-hGHpA sequence. Total insert size including the two ITRs is 5028 bp.
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: tdTomato
• Insert Size (bp): 1461
• Promoter: pCAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Not1 (not destroyed)
• 3′ cloning site: Not1 (not destroyed)
• 5′ sequencing primer: none
• 3′ sequencing primer: none

Resource Information

• Articles Citing this Plasmid:
  • 24 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

NOTE: The map generated by Addgene's software is inaccurate. ORF 3 is identified as dTomato, but the entire ORF 2 is actually a codon-optimized version of tdTomato.