pET28a-ClbN-NHis
(Plasmid
#51497)
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PurposeNRPS module containing CATE domains with N-terminal 6His tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51497 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-28 a (+)
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5368
- Total vector size (bp) 9717
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameClbN
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SpeciesEscherichia coli CFT073
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Mutationcontains aa30-1094 of NP_754347.1
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GenBank IDNP_754347.1
- Promoter T7
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Tags
/ Fusion Proteins
- 6x His tag (N terminal on insert)
- T7 tag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CAGATCGAATTCATGATGTCGGGCAATCCGCAGATCGAATTCATGATGTCGGGCAATCCG
- 3′ sequencing primer ATTAGCGGCCGCTCATAGTGTCCACAAAGTC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a-ClbN-NHis was a gift from Emily Balskus (Addgene plasmid # 51497 ; http://n2t.net/addgene:51497 ; RRID:Addgene_51497) -
For your References section:
A prodrug resistance mechanism is involved in colibactin biosynthesis and cytotoxicity. Brotherton CA, Balskus EP. J Am Chem Soc. 2013 Mar 6;135(9):3359-62. doi: 10.1021/ja312154m. Epub 2013 Feb 20. 10.1021/ja312154m PubMed 23406518
Map uploaded by the depositor.
