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Addgene

pCFJ1666
(Plasmid #51485)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51485 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDESTR4-R3
  • Backbone manufacturer
    Invitrogen
  • Backbone size (bp) 2400
  • Modifications to backbone
    Insertion for PuroR
  • Vector type
    Worm Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Cloning Information

  • Cloning method Unknown

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Puromycin selection developed by Ben Lehner's lab. Please see Semple et al. (2010) for details.
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See www.wormbuilder.org for updated protocols.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCFJ1666 was a gift from Erik Jorgensen (Addgene plasmid # 51485 ; http://n2t.net/addgene:51485 ; RRID:Addgene_51485)

  • For your References section:

    Random and targeted transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon. Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella M, Pozniakovsky A, Moerman DG, Jorgensen EM. Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 2014 Mar 16. 10.1038/nmeth.2889 PubMed 24820376
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