pCFJ1662
(Plasmid #51482)

• Purpose: (Empty Backbone) miniMos transposable element with HygroR, MCS cloning
• Depositing Lab: Erik Jorgensen
• Publication: Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 2014 Mar 16.

Backbone

• Vector backbone: pDESTR4-R3
• Backbone manufacturer: Invitrogen
• Backbone size (bp): 2400
• Modifications to backbone: Insertion of HygroR and MCS
• Vector type: Worm Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Growth instructions: Important to use dual selection to limit recombination of att sites.
• Copy number: High Copy

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: HygroR derived from plasmid from Jason Chin's lab (Cambridge, UK). See Radman et al. (2013) publication for details on hygromycin B selection.
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See www.wormbuilder.org for updated protocols.