pCFJ1655
(Plasmid
#51481)
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Purpose(Empty Backbone) miniMos transposable element with HygroR, R4-R3 Gateway cloning
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51481 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDESTR4-R3
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Backbone manufacturerInvitrogen
- Backbone size (bp) 2400
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Modifications to backboneInsertion of HygroR
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Vector typeWorm Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Growth instructionsImportant to use dual selection to limit recombination of att sites.
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Copy numberHigh Copy
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byHygroR derived from plasmid from Jason Chin's lab (Cambridge, UK). See Radman et al. (2013) publication for details on hygromycin B selection.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See www.wormbuilder.org for updated protocols.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCFJ1655 was a gift from Erik Jorgensen (Addgene plasmid # 51481 ; http://n2t.net/addgene:51481 ; RRID:Addgene_51481) -
For your References section:
Random and targeted transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon. Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella M, Pozniakovsky A, Moerman DG, Jorgensen EM. Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 2014 Mar 16. 10.1038/nmeth.2889 PubMed 24820376
Map uploaded by the depositor.
