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Purpose(Empty Backbone) mammalian cell expression vector
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51476 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSTC
- Backbone size (bp) 5000
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Modifications to backbonepCG is a derivative of pSTC described by Severne, Y., Wieland, S., Schaffner. W., and Rusconi. S. (1988). EMBO J. 7: 2503-2508. To create pCG from pSTC an M13 origin of replication was inserted, a unique XbaI site created, and a polylinker inserted after removal of glucocorticoid receptor gene sequences.
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCG was a gift from Winship Herr (Addgene plasmid # 51476 ; http://n2t.net/addgene:51476 ; RRID:Addgene_51476) -
For your References section:
Differential transcriptional activation by Oct-1 and Oct-2: interdependent activation domains induce Oct-2 phosphorylation. Tanaka M, Herr W. Cell. 1990 Feb 9;60(3):375-86. 10.1016/0092-8674(90)90589-7 PubMed 2302733
Map uploaded by the depositor.
