GFP-P4M-SidMx2
(Plasmid #51472)

• Purpose: Biosensor for PtdIns4P
• Depositing Lab: Tamas Balla
• Publication: Hammond et al J Cell Biol. 2014 Apr 7.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: P4M-SidM(546-647)
• Species: Legionella pneumophila
• Insert Size (bp): 321
• GenBank ID: DQ845395.1
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40-R

Gene/Insert 2

• Gene/Insert name: P4M-SidM(546-647)
• Species: Legionella pneumophila
• Insert Size (bp): 321
• GenBank ID: DQ845395.1
• Promoter: CMV

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40-R

Resource Information

• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry