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Addgene

iRFP-P4M-SidM
(Plasmid #51470)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51470 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    piRFP-N1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    P4M-SidM(546-647)
  • Species
    Legionella pneumophila
  • Insert Size (bp)
    321
  • GenBank ID
    DQ845395.1
  • Promoter CMV
  • Tag / Fusion Protein
    • iRFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    iRFP-P4M-SidM was a gift from Tamas Balla (Addgene plasmid # 51470 ; http://n2t.net/addgene:51470 ; RRID:Addgene_51470)

  • For your References section:

    A novel probe for phosphatidylinositol 4-phosphate reveals multiple pools beyond the Golgi. Hammond GR, Machner MP, Balla T. J Cell Biol. 2014 Apr 7. 10.1083/jcb.201312072 PubMed 24711504
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