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Addgene

pJ207 Delta-cpc-KanR
(Plasmid #51468)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51468 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJ207
  • Backbone manufacturer
    DNA2.0
  • Backbone size w/o insert (bp) 4835
  • Total vector size (bp) 6763
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    None
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Replacing the cpc operon with kanamycin resistance
  • Alt name
    Delta-cpc-KanR
  • Species
    Synthetic
  • Insert Size (bp)
    1928
  • Promoter Synechocystis endogenous cpc-operon promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII restriction site (not destroyed)
  • 3′ cloning site SacI restriction site (not destroyed)
  • 5′ sequencing primer None
  • 3′ sequencing primer None
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Formighieri C, Melis A (2015) A phycocyanin·phellandrene synthase fusion enhances recombinant protein expression and β-phellandrene (monoterpene) hydrocarbons production in Synechocystis (cyanobacteria). Metab Eng 32:116–124. http://dx.doi.org/10.1016/j.ymben.2015.09.010

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJ207 Delta-cpc-KanR was a gift from Anastasios Melis (Addgene plasmid # 51468 ; http://n2t.net/addgene:51468 ; RRID:Addgene_51468)

  • For your References section:

    Maximizing photosynthetic efficiency and culture productivity in cyanobacteria upon minimizing the phycobilisome light-harvesting antenna size. Kirst H, Formighieri C, Melis A. Biochim Biophys Acta. 2014 Oct;1837(10):1653-64. doi: 10.1016/j.bbabio.2014.07.009. Epub 2014 Jul 18. 10.1016/j.bbabio.2014.07.009 PubMed 25046143
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