PH-Btk(R28C)-GFP
(Plasmid #51464)

• Purpose: Non-PIP3 binding control for PH-Btk-GFP
• Depositing Lab: Tamas Balla
• Publication: Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Total vector size (bp): 5253
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BTK (aa 1–177)
• Species: H. sapiens (human)
• Insert Size (bp): 531
• Mutation: R28C
• GenBank ID: NM_000061.2
• Entrez Gene: BTK (a.k.a. AGMX1, AT, ATK, BPK, IGHD3, IMD1, PSCTK1, XLA)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-N

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry