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Tol2-mpeg1-dendra2
(Plasmid #51462)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51462 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    tol2
  • Backbone size w/o insert (bp) 3500
  • Total vector size (bp) 6000
  • Modifications to backbone
    inserted mpeg promoter + dendra2
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mpeg-dendra
  • Insert Size (bp)
    2500
  • Promoter mpeg1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer Gtgaaagtacaagtacttagggaaaa
  • 3′ sequencing primer gtgtggaattgtgagcggataac
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tol2-mpeg1-dendra2 was a gift from Anna Huttenlocher (Addgene plasmid # 51462 ; http://n2t.net/addgene:51462 ; RRID:Addgene_51462)

  • For your References section:

    Innate immune response to Streptococcus iniae infection in zebrafish larvae. Harvie EA, Green JM, Neely MN, Huttenlocher A. Infect Immun. 2013 Jan;81(1):110-21. doi: 10.1128/IAI.00642-12. Epub 2012 Oct 22. 10.1128/IAI.00642-12 PubMed 23090960
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