pHD-DsRed
(Plasmid #51434)

• Purpose: Vector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed.
• Depositing Lab: Kate O'Connor-Giles
• Publication: Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 10.1534/genetics.113.160713. Epub 2014 Jan 29.

Backbone

• Vector backbone: pJ204
• Backbone manufacturer: DNA 2.0
• Backbone size w/o insert (bp): 2703
• Total vector size (bp): 4048

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LoxP-3xP3-DsRed-LoxP
• Species: Synthetic
• Insert Size (bp): 1225
• Promoter: 3xP3

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: NA
• 3′ sequencing primer: NA

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We had it synthesized by DNA2.0.
• Articles Citing this Plasmid:
  • 37 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry