pGolden-Hyg
(Plasmid #51423)

• Purpose: Contain one loxP-PGK-Hyg-pA-loxP cassette, used for golden-gate reaction
• Depositing Lab: Yonglun Luo
• Publication: Luo et al Biotechniques. 2014 May 1;56(5):263-8. doi: 10.2144/000114169. eCollection 2014 May.

Backbone

• Vector backbone: pTopo
• Backbone manufacturer: lifetechnologies
• Backbone size w/o insert (bp): 3519
• Total vector size (bp): 5756
• Modifications to backbone: no
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PGK-Hyg-pA
• Species: H. sapiens (human)
• Insert Size (bp): 2235
• Entrez Gene: PGK1 (a.k.a. HEL-S-68p, MIG10, PGKA)
• Promoter: PGK

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: GGAAACAGCTATGACCATG
• 3′ sequencing primer: GTAAAACGACGGCCAGT

Resource Information

• Supplemental Documents:
  • annotated sequence file
• A portion of this plasmid was derived from a plasmid made by: The PGK-Neo-pA cassette was PCR amplified from the pNeDaKo-Hyg vector from Bert Vogelstein and Kenneth W. Kinzler, John Hopkins University, Baltimore, US
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry