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Addgene

CMV-p150
(Plasmid #51412)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51412 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGW1-CMV
  • Backbone size w/o insert (bp) 4800
  • Total vector size (bp) 8900
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DCTN1
  • Alt name
    p150Glued
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    4100
  • Entrez Gene
    DCTN1 (a.k.a. Chip150)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer None
  • 3′ sequencing primer None
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-p150 was a gift from Trina Schroer (Addgene plasmid # 51412 ; http://n2t.net/addgene:51412 ; RRID:Addgene_51412)
  • For your References section:

    Dynactin is required for microtubule anchoring at centrosomes. Quintyne NJ, Gill SR, Eckley DM, Crego CL, Compton DA, Schroer TA. J Cell Biol. 1999 Oct 18;147(2):321-34. 10.1083/jcb.147.2.321 PubMed 10525538