EGFP-IC2-FL
(Plasmid #51409)

• Purpose: expression of EGFP-tagged full-length dynein intermediate chain IC2C in mammalian cells
• Depositing Lab: Trina Schroer
• Publication: King et al Mol Biol Cell. 2003 Dec;14(12):5089-97. Epub 2003 Oct 17.

Backbone

• Vector backbone: pEGFP-C2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4731
• Total vector size (bp): 6500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dynein intermediate chain IC2C
• Alt name: Dync1i2
• Species: M. musculus (mouse)
• Insert Size (bp): 1730
• Entrez Gene: Dync1i2 (a.k.a. 3110079H08Rik, AW554389, Dnci, Dncic2)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: None
• 3′ sequencing primer: NONE

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry