PH-PLCD1(R40L)-GFP
(Plasmid #51408)

• Purpose: Non-PI(4,5)P2 binding control for PH-PLCD1-GFP
• Depositing Lab: Tamas Balla
• Publication: Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Total vector size (bp): 5520
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PH-PLCD1 (aa 1–170)
• Species: H. sapiens (human)
• Insert Size (bp): 510
• Mutation: Arginine 40 mutated to leucine
• GenBank ID: NP_006216.2
• Entrez Gene: PLCD1 (a.k.a. NDNC3, PLC-III)
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The S34T mutation found during the QC process has no functional consequence.