pcDNA3.1(+)IRES GFP
(Plasmid #51406)

• Purpose: Expresses GFP from internal ribosome entry sequence, cloning component
• Depositing Lab: Kathleen_L Collins
• Publication: Schaefer et al J Immunol. 2008 Jun 15;180(12):7804-17.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Life Technologies
• Backbone size w/o insert (bp): 5440
• Total vector size (bp): 6772
• Modifications to backbone: none
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: IRES GFP
• Alt name: internal ribosome entry site
• Alt name: green fluorescent protein
• Insert Size (bp): 1332
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XhoI (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH reverse

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 18 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The discrepancies between the Addgene QC sequence and the full sequence have no functional consequence.