T7-His-Arp11(1–417)
(Plasmid
#51400)
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Purposeexpression of full-length mouse Arp11 under T7 promoter
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51400 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRSET-B
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2900
- Total vector size (bp) 4444
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameArp11
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Alt nameActr10
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SpeciesM. musculus (mouse)
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Insert Size (bp)1251
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Entrez GeneActr10 (a.k.a. Actr11, Arp11)
- Promoter T7
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Tag
/ Fusion Protein
- His (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site NcoI (not destroyed)
- 5′ sequencing primer None
- 3′ sequencing primer None (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
T7-His-Arp11(1–417) was a gift from Trina Schroer (Addgene plasmid # 51400 ; http://n2t.net/addgene:51400 ; RRID:Addgene_51400) -
For your References section:
Interactions between the evolutionarily conserved, actin-related protein, Arp11, actin, and Arp1. Eckley DM, Schroer TA. Mol Biol Cell. 2003 Jul;14(7):2645-54. Epub 2003 Mar 20. 10.1091/mbc.e03-01-0049 PubMed 12857853