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Addgene

GFP-fly Arp11
(Plasmid #51399)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51399 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5860
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Arp11
  • Alt name
    Actr10
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1136
  • Entrez Gene
    Arp10 (a.k.a. Dmel_CG12235, ARP11, Arp11, CG12235, Dmel\CG12235)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer None
  • 3′ sequencing primer None
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-fly Arp11 was a gift from Trina Schroer (Addgene plasmid # 51399 ; http://n2t.net/addgene:51399 ; RRID:Addgene_51399)

  • For your References section:

    Interactions between the evolutionarily conserved, actin-related protein, Arp11, actin, and Arp1. Eckley DM, Schroer TA. Mol Biol Cell. 2003 Jul;14(7):2645-54. Epub 2003 Mar 20. 10.1091/mbc.e03-01-0049 PubMed 12857853
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