GFP-Arp11 (1-417)
(Plasmid #51398)

• Purpose: expression of full-length mouse Arp11 in mammalian cells
• Depositing Lab: Trina Schroer
• Publication: Eckley et al Mol Biol Cell. 2003 Jul;14(7):2645-54. Epub 2003 Mar 20.

Backbone

• Vector backbone: pEGFP-C2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5953
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Arp11
• Alt name: Actr10
• Species: M. musculus (mouse)
• Insert Size (bp): 1253
• Entrez Gene: Actr10 (a.k.a. Actr11, Arp11)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: None
• 3′ sequencing primer: None

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry