KAP3-CT
(Plasmid #51396)

• Purpose: expression of EGFP-tagged COOH-terminal AA 661–793 KAP3B in mammalian cells
• Depositing Lab: Trina Schroer
• Publication: Brown et al Traffic. 2005 Dec;6(12):1114-24.

Backbone

• Vector backbone: pEGFP-C3
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5100
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Kinesin-associated protein 3
• Alt name: KAP3B
• Species: H. sapiens (human)
• Insert Size (bp): 400
• GenBank ID: NM_001204514
• Entrez Gene: KIFAP3 (a.k.a. FLA3, KAP-1, KAP-3, KAP3, SMAP, Smg-GDS, dJ190I16.1)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: GATTATCTCGAGGAATATGATGAAGAATG
• 3′ sequencing primer: CTAATAGAATTCTTATCAAGATCCATAG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry