pGL3-CMV-p85a 3'UTR mutant
(Plasmid
#51393)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51393 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGL3-UC
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Backbone manufacturerPromega (modified to have 3' multi cloning sites)
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep85alpha 3'UTR
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SpeciesH. sapiens (human)
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MutationmiR-29 binding site mutant
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Entrez GenePIK3R1 (a.k.a. AGM7, GRB1, IMD36, p85, p85-ALPHA, p85alpha)
- Promoter CMV
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Tag
/ Fusion Protein
- Luciferase (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer Luc-F
- 3′ sequencing primer EBV_rev_primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
miR-29 seed mutant (TGCT->GTAG)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL3-CMV-p85a 3'UTR mutant was a gift from Narry Kim (Addgene plasmid # 51393 ; http://n2t.net/addgene:51393 ; RRID:Addgene_51393) -
For your References section:
miR-29 miRNAs activate p53 by targeting p85 alpha and CDC42. Park SY, Lee JH, Ha M, Nam JW, Kim VN. Nat Struct Mol Biol. 2009 Jan;16(1):23-9. doi: 10.1038/nsmb.1533. Epub 2008 Dec 14. 10.1038/nsmb.1533 PubMed 19079265