pGL3-23P639
(Plasmid #51388)

• Depositing Lab: Narry Kim
• Publication: Lee et al EMBO J. 2004 Oct 13;23(20):4051-60. Epub 2004 Sep 16.

Backbone

• Vector backbone: pGL3-basic
• Backbone manufacturer: Promega
• Vector type: Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: miR-23a promoter region
• Species: H. sapiens (human)
• Entrez Gene: MIR23A (a.k.a. MIRN23A, hsa-mir-23a, miRNA23A, mir-23a)
• Tag / Fusion Protein:
  • Luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: RVprimer3_primer
• 3′ sequencing primer: LucNrev_primer

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

position: -603~+36nt (compared to transcription start site of pri-miR-23a) (sequence: Fig. 4A)

Addgene sequencing has found a T50260C and A50093 insert relative to human chromosome 19 (gb|AC020916.8), this is in the promoter region and is not believed to effect the function of the plasmid.