pcDNA3-V5-DGCR8-mDRBD1&2
(Plasmid
#51385)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51385 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDGCR8
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SpeciesH. sapiens (human)
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MutationDRBD1 & DRBD2 mutant (A568K, A569K, A676K, S677K)
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Entrez GeneDGCR8 (a.k.a. C22orf12, DGCRK6, Gy1, pasha)
- Promoter CMV
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Tag
/ Fusion Protein
- V5 (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CAATGGGCGTGGATAGCG
- 3′ sequencing primer CCAGGGTCAAGGAAGGCAC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-V5-DGCR8-mDRBD1&2 was a gift from Narry Kim (Addgene plasmid # 51385 ; http://n2t.net/addgene:51385 ; RRID:Addgene_51385) -
For your References section:
Characterization of DGCR8/Pasha, the essential cofactor for Drosha in primary miRNA processing. Yeom KH, Lee Y, Han J, Suh MR, Kim VN. Nucleic Acids Res. 2006;34(16):4622-9. Epub 2006 Sep 8. 10.1093/nar/gkl458 PubMed 16963499