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Addgene

pcDNA3-miR-29b-1~29a
(Plasmid #51376)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51376 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pri-miR-29b-1~29a
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1118
  • Entrez Gene
    MIR29B1 (a.k.a. MIRN29B1, miR-29b, miRNA29B1, mir-29b-1)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CAATGGGCGTGGATAGCG
  • 3′ sequencing primer CCAGGGTCAAGGAAGGCAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Point mutations: insertion (1115bp), deletion (A47, T666) (Unnecessary region for miRNA biogenesis)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-miR-29b-1~29a was a gift from Narry Kim (Addgene plasmid # 51376 ; http://n2t.net/addgene:51376 ; RRID:Addgene_51376)

  • For your References section:

    miR-29 miRNAs activate p53 by targeting p85 alpha and CDC42. Park SY, Lee JH, Ha M, Nam JW, Kim VN. Nat Struct Mol Biol. 2009 Jan;16(1):23-9. doi: 10.1038/nsmb.1533. Epub 2008 Dec 14. 10.1038/nsmb.1533 PubMed 19079265
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