pcDNA3-FLAG-Lin28B-mCCHC
(Plasmid
#51375)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51375 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameLin28B
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SpeciesH. sapiens (human)
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MutationCCHC domain mutant (H137A, H159A)
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Entrez GeneLIN28B (a.k.a. CSDD2)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CAATGGGCGTGGATAGCG
- 3′ sequencing primer CCAGGGTCAAGGAAGGCAC (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
H137A, H179A
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-FLAG-Lin28B-mCCHC was a gift from Narry Kim (Addgene plasmid # 51375 ; http://n2t.net/addgene:51375 ; RRID:Addgene_51375) -
For your References section:
TUT4 in concert with Lin28 suppresses microRNA biogenesis through pre-microRNA uridylation. Heo I, Joo C, Kim YK, Ha M, Yoon MJ, Cho J, Yeom KH, Han J, Kim VN. Cell. 2009 Aug 21;138(4):696-708. doi: 10.1016/j.cell.2009.08.002. 10.1016/j.cell.2009.08.002 PubMed 19703396