pcDNA3-FLAG-Lin28A-mCCHC
(Plasmid #51372)

• Depositing Lab: Narry Kim
• Publication: Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi: 10.1016/j.molcel.2008.09.014.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Lin28A
• Alt name: Lin28
• Species: H. sapiens (human)
• Insert Size (bp): 630
• Mutation: CCHC domain mutant (H147A, H169A)
• Entrez Gene: LIN28A (a.k.a. CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CAATGGGCGTGGATAGCG
• 3′ sequencing primer: CCAGGGTCAAGGAAGGCAC

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

H147A, H169A