pcDNA3.1-FLAG-Dicer-delHelicC
(Plasmid #51366)

• Depositing Lab: Narry Kim
• Publication: Lee et al EMBO J. 2006 Feb 8;25(3):522-32. Epub 2006 Jan 19.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Dicer
• Alt name: Dicer1
• Species: H. sapiens (human)
• Mutation: From N terminus to HelicC domain deletion
• Entrez Gene: DICER1 (a.k.a. DCR1, Dicer, Dicer1e, GLOW, HERNA, K12H4.8-LIKE, MNG1, RMSE2, aviD)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: ApaI (not destroyed)
• 5′ sequencing primer: CMV_fwd_primer
• 3′ sequencing primer: BGH_rev_primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Note that the full-length wild type Dicer was cloned by Dr. Patrick Provost (PMID: 12411504).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry