pcDNA3.1-FLAG-Dicer-delDEAD
(Plasmid
#51364)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 51364 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDicer
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Alt nameDicer1
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SpeciesH. sapiens (human)
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MutationFrom N terminus to DEAD domain deletion
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Entrez GeneDICER1 (a.k.a. DCR1, Dicer, Dicer1e, GLOW, HERNA, K12H4.8-LIKE, MNG1, RMSE2, aviD)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site ApaI (not destroyed)
- 5′ sequencing primer CMV_fwd_primer
- 3′ sequencing primer BGH_rev_primer (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byNote that the full-length wild type Dicer was cloned by Dr. Patrick Provost (PMID: 12411504).
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-FLAG-Dicer-delDEAD was a gift from Narry Kim (Addgene plasmid # 51364 ; http://n2t.net/addgene:51364 ; RRID:Addgene_51364) -
For your References section:
The role of PACT in the RNA silencing pathway. Lee Y, Hur I, Park SY, Kim YK, Suh MR, Kim VN. EMBO J. 2006 Feb 8;25(3):522-32. Epub 2006 Jan 19. 10.1038/sj.emboj.7600942 PubMed 16424907