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Addgene

pcDNA3.1-FLAG-Dicer-delDEAD
(Plasmid #51364)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51364 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dicer
  • Alt name
    Dicer1
  • Species
    H. sapiens (human)
  • Mutation
    From N terminus to DEAD domain deletion
  • Entrez Gene
    DICER1 (a.k.a. DCR1, Dicer, Dicer1e, GLOW, HERNA, K12H4.8-LIKE, MNG1, RMSE2, aviD)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer CMV_fwd_primer
  • 3′ sequencing primer BGH_rev_primer
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Note that the full-length wild type Dicer was cloned by Dr. Patrick Provost (PMID: 12411504).
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-FLAG-Dicer-delDEAD was a gift from Narry Kim (Addgene plasmid # 51364 ; http://n2t.net/addgene:51364 ; RRID:Addgene_51364)
  • For your References section:

    The role of PACT in the RNA silencing pathway. Lee Y, Hur I, Park SY, Kim YK, Suh MR, Kim VN. EMBO J. 2006 Feb 8;25(3):522-32. Epub 2006 Jan 19. 10.1038/sj.emboj.7600942 PubMed 16424907