4IA9
(Plasmid
#51320)
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PurposeBacterial expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51320 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepET28-MHL
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameWDR5
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Alt namePDB ID: 4IA9, 3SMR, 4QQE, 8T5I
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Alt namegi|61744459
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Alt nameWDR5:APC041-G05:C36415
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SpeciesH. sapiens (human)
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MutationTruncation: See Comments
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GenBank ID
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Entrez GeneWDR5 (a.k.a. BIG-3, BIG3, CFAP89, SWD3)
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
4IA9 was a gift from Cheryl Arrowsmith (Addgene plasmid # 51320 ; http://n2t.net/addgene:51320 ; RRID:Addgene_51320)