4HSG
(Plasmid
#51318)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 51318 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a-LIC
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name4HSG
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Alt nameHRMT1L3
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Alt namegi|44771197
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SpeciesH. sapiens (human)
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MutationTruncation: See Comments
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GenBank IDHRMT1L3
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Entrez GenePRMT3 (a.k.a. HRMT1L3)
- Promoter T7
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Tag
/ Fusion Protein
- His (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
4HSG was a gift from Cheryl Arrowsmith (Addgene plasmid # 51318 ; http://n2t.net/addgene:51318 ; RRID:Addgene_51318)