4HC4
(Plasmid
#51317)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 51317 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepFBOH-MHL
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Vector typebaculovirus expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name4HC4
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Alt nameHRMT1L6
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Alt namegi|8922514
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SpeciesH. sapiens (human)
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MutationTruncation: See Comments
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Entrez GenePRMT6 (a.k.a. HRMT1L6)
- Promoter T7
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Tag
/ Fusion Protein
- His (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
http://www.thesgc.org/structures/4HC4/
Additional information regarding the construct can be found at http://www.rcsb.org/structure/6w6d and http://www.rcsb.org/structure/6WAD
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
4HC4 was a gift from Cheryl Arrowsmith (Addgene plasmid # 51317 ; http://n2t.net/addgene:51317 ; RRID:Addgene_51317)