pMath3-luc
(Plasmid #51312)

• Purpose: Luciferase reporter driven by the Math3 promoter
• Depositing Lab: Ryoichiro Kageyama
• Publication: Tsuda et al J Biol Chem. 1998 Mar 13;273(11):6327-33.

Backbone

• Vector backbone: Pica Gene basic vector
• Total vector size (bp): 5597
• Vector type: Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Math3 promoter (-2.8k- +196)
• Species: M. musculus (mouse)
• Insert Size (bp): 3000
• GenBank ID: AF034778.1
• Entrez Gene: Neurod4 (a.k.a. ATH-3, Atoh3, MATH-3, Math3, bHLHa4)
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HincII (destroyed during cloning)
• 3′ cloning site: SmaI (destroyed during cloning)
• 5′ sequencing primer: F1ori-F
• 3′ sequencing primer: LucNRev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry