pcDNA3-mNeptune2.5
(Plasmid #51310)

• Purpose: Expresses far-red fluorescent protein mNeptune2.5 in mammalian cells
• Depositing Lab: Michael Lin
• Publication: Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5425
• Total vector size (bp): 6157
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mNeptune2.5
• Species: Synthetic; sea anemone Entacmaea quadricolor
• Insert Size (bp): 732
• GenBank ID: KJ131554
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: sp6

Resource Information

• Supplemental Documents:
  • pcDNA3-mNeptune2.5.gb
• Addgene Notes:
  • Addgene Diagnostic Test
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry