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Addgene

Pyronic/pcDNA3.1(-)
(Plasmid #51308)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51308 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1(-)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5419
  • Total vector size (bp) 7681
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418), Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pyronic
  • Species
    Synthetic
  • Insert Size (bp)
    2262
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7Promoter
  • 3′ sequencing primer BGH Reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Pyronic/pcDNA3.1(-) was a gift from Luis Felipe Barros (Addgene plasmid # 51308 ; http://n2t.net/addgene:51308 ; RRID:Addgene_51308)
  • For your References section:

    Imaging Mitochondrial Flux in Single Cells with a FRET Sensor for Pyruvate. San Martin A, Ceballo S, Baeza-Lehnert F, Lerchundi R, Valdebenito R, Contreras-Baeza Y, Alegria K, Barros LF. PLoS One. 2014 Jan 21;9(1):e85780. doi: 10.1371/journal.pone.0085780. eCollection 2014 Jan 21. 10.1371/journal.pone.0085780 PubMed 24465702