Pyronic/pcDNA3.1(-)
(Plasmid #51308)

• Purpose: Pyronic, FRET sensor that allow quantitative measurement of pyruvate transport, pyruvate production and mitochondrial pyruvate consumption in intact individual cells at high temporal resolution.
• Depositing Lab: Luis Felipe Barros
• Publication: San Martin et al PLoS One. 2014 Jan 21;9(1):e85780. doi: 10.1371/journal.pone.0085780. eCollection 2014 Jan 21.

Backbone

• Vector backbone: pcDNA3.1(-)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5419
• Total vector size (bp): 7681
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418), Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Pyronic
• Species: Synthetic
• Insert Size (bp): 2262
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7Promoter
• 3′ sequencing primer: BGH Reverse

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry