pCS2-3xFLAG-NLS-SpCas9-NLS Citations (18)
Originally described in: Efficient RNA/Cas9-mediated genome editing in Xenopus tropicalis.Guo X, Zhang T, Hu Z, Zhang Y, Shi Z, Wang Q, Cui Y, Wang F, Zhao H, Chen Y Development. 2014 Jan 8. PubMed Journal
Articles Citing pCS2-3xFLAG-NLS-SpCas9-NLS
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Differential levels of Neurod establish zebrafish endocrine pancreas cell fates. Dalgin G, Prince VE. Dev Biol. 2015 Jun 1;402(1):81-97. doi: 10.1016/j.ydbio.2015.03.007. Epub 2015 Mar 20. PubMed |
Albinism as a visual, in vivo guide for CRISPR/Cas9 functionality in the sea urchin embryo. Oulhen N, Wessel GM. Mol Reprod Dev. 2016 Dec;83(12):1046-1047. doi: 10.1002/mrd.22757. Epub 2016 Nov 30. PubMed |
Transient translational quiescence in primordial germ cells. Oulhen N, Swartz SZ, Laird J, Mascaro A, Wessel GM. Development. 2017 Apr 1;144(7):1201-1210. doi: 10.1242/dev.144170. Epub 2017 Feb 24. PubMed |
Mutation of the Sp1 binding site in the 5' flanking region of SRY causes sex reversal in rabbits. Song Y, Liu T, Wang Y, Deng J, Chen M, Yuan L, Lu Y, Xu Y, Yao H, Li Z, Lai L. Oncotarget. 2017 Jun 13;8(24):38176-38183. doi: 10.18632/oncotarget.16979. PubMed |
CRISPR/Cas9-Mediated Mutation of alphaA-Crystallin Gene Induces Congenital Cataracts in Rabbits. Yuan L, Yao H, Xu Y, Chen M, Deng J, Song Y, Sui T, Wang Y, Huang Y, Li Z, Lai L. Invest Ophthalmol Vis Sci. 2017 May 1;58(6):BIO34-BIO41. doi: 10.1167/iovs.16-21287. PubMed |
Single nucleotide editing without DNA cleavage using CRISPR/Cas9-deaminase in the sea urchin embryo. Shevidi S, Uchida A, Schudrowitz N, Wessel GM, Yajima M. Dev Dyn. 2017 Dec;246(12):1036-1046. doi: 10.1002/dvdy.24586. Epub 2017 Oct 13. PubMed |
CRISPR/Cas9-mediated mosaic mutation of SRY gene induces hermaphroditism in rabbits. Song Y, Xu Y, Liang M, Zhang Y, Chen M, Deng J, Li Z. Biosci Rep. 2018 Mar 21;38(2). pii: BSR20171490. doi: 10.1042/BSR20171490. Print 2018 Apr 27. PubMed |
Expanded targeting scope and enhanced base editing efficiency in rabbit using optimized xCas9(3.7). Liu Z, Chen M, Shan H, Chen S, Xu Y, Song Y, Zhang Q, Yuan H, Ouyang H, Li Z, Lai L. Cell Mol Life Sci. 2019 Apr 27. pii: 10.1007/s00018-019-03110-8. doi: 10.1007/s00018-019-03110-8. PubMed |
Distinct transcriptional regulation of Nanos2 in the germ line and soma by the Wnt and delta/notch pathways. Oulhen N, Swartz SZ, Wang L, Wikramanayake A, Wessel GM. Dev Biol. 2019 Aug 1;452(1):34-42. doi: 10.1016/j.ydbio.2019.04.010. Epub 2019 May 7. PubMed |
Genetic deletion of a short fragment of glucokinase in rabbit by CRISPR/Cas9 leading to hyperglycemia and other typical features seen in MODY-2. Song Y, Sui T, Zhang Y, Wang Y, Chen M, Deng J, Chai Z, Lai L, Li Z. Cell Mol Life Sci. 2020 Aug;77(16):3265-3277. doi: 10.1007/s00018-019-03354-4. Epub 2019 Nov 13. PubMed |
CRISPR-Cas9-Mutated Pregnane X Receptor (pxr) Retains Pregnenolone-induced Expression of cyp3a65 in Zebrafish (Danio rerio) Larvae. Salanga MC, Brun NR, Francolini RD, Stegeman JJ, Goldstone JV. Toxicol Sci. 2020 Mar 1;174(1):51-62. doi: 10.1093/toxsci/kfz246. PubMed |
Genetic manipulation of the pigment pathway in a sea urchin reveals distinct lineage commitment prior to metamorphosis in the bilateral to radial body plan transition. Wessel GM, Kiyomoto M, Shen TL, Yajima M. Sci Rep. 2020 Feb 6;10(1):1973. doi: 10.1038/s41598-020-58584-5. PubMed |
Midline morphogenesis of zebrafish foregut endoderm is dependent on Hoxb5b. Dalgin G, Prince VE. Dev Biol. 2021 Mar;471:1-9. doi: 10.1016/j.ydbio.2020.12.001. Epub 2020 Dec 5. PubMed |
Sperm lacking Bindin are infertile but are otherwise indistinguishable from wildtype sperm. Wessel GM, Wada Y, Yajima M, Kiyomoto M. Sci Rep. 2021 Nov 3;11(1):21583. doi: 10.1038/s41598-021-00570-6. PubMed |
Orphan cytochrome P450 20a1 CRISPR/Cas9 mutants and neurobehavioral phenotypes in zebrafish. Brun NR, Salanga MC, Mora-Zamorano FX, Lamb DC, Goldstone JV, Stegeman JJ. Sci Rep. 2021 Dec 13;11(1):23892. doi: 10.1038/s41598-021-03068-3. PubMed |
CRISPR/Cas9-Mediated Models of Retinitis Pigmentosa Reveal Differential Proliferative Response of Muller Cells between Xenopus laevis and Xenopus tropicalis. Parain K, Lourdel S, Donval A, Chesneau A, Borday C, Bronchain O, Locker M, Perron M. Cells. 2022 Feb 25;11(5). pii: cells11050807. doi: 10.3390/cells11050807. PubMed |
YIPF5 (p.W218R) mutation induced primary microcephaly in rabbits. Liu X, Yang J, Li Z, Liu R, Wu X, Zhang Z, Lai L, Li Z, Song Y. Neurobiol Dis. 2023 Jun 15;182:106135. doi: 10.1016/j.nbd.2023.106135. Epub 2023 May 2. PubMed |
Molecular mechanisms of tubulogenesis revealed in the sea star hydro-vascular organ. Perillo M, Swartz SZ, Pieplow C, Wessel GM. Nat Commun. 2023 May 9;14(1):2402. doi: 10.1038/s41467-023-37947-2. PubMed |
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