pUC57-Simple-gRNA backbone Citations (21)

Originally described in: Efficient RNA/Cas9-mediated genome editing in Xenopus tropicalis.
Guo X, Zhang T, Hu Z, Zhang Y, Shi Z, Wang Q, Cui Y, Wang F, Zhao H, Chen Y Development. 2014 Jan 8. PubMed Journal

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Articles Citing pUC57-Simple-gRNA backbone

Articles
Efficient dual sgRNA-directed large gene deletion in rabbit with CRISPR/Cas9 system. Song Y, Yuan L, Wang Y, Chen M, Deng J, Lv Q, Sui T, Li Z, Lai L. Cell Mol Life Sci. 2016 Aug;73(15):2959-68. doi: 10.1007/s00018-016-2143-z. Epub 2016 Jan 27. PubMed
CRISPR/Cas9-mediated GJA8 knockout in rabbits recapitulates human congenital cataracts. Yuan L, Sui T, Chen M, Deng J, Huang Y, Zeng J, Lv Q, Song Y, Li Z, Lai L. Sci Rep. 2016 Feb 25;6:22024. doi: 10.1038/srep22024. PubMed
Efficient Generation of Myostatin Gene Mutated Rabbit by CRISPR/Cas9. Lv Q, Yuan L, Deng J, Chen M, Wang Y, Zeng J, Li Z, Lai L. Sci Rep. 2016 Apr 26;6:25029. doi: 10.1038/srep25029. PubMed
CRISPR/Cas9-mediated mutation of PHEX in rabbit recapitulates human X-linked hypophosphatemia (XLH). Sui T, Yuan L, Liu H, Chen M, Deng J, Wang Y, Li Z, Lai L. Hum Mol Genet. 2016 Apr 28. pii: ddw125. PubMed
CRISPR/Cas9-mediated mutation of tyrosinase (Tyr) 3' UTR induce graying in rabbit. Song Y, Xu Y, Deng J, Chen M, Lu Y, Wang Y, Yao H, Zhou L, Liu Z, Lai L, Li Z. Sci Rep. 2017 May 8;7(1):1569. doi: 10.1038/s41598-017-01727-y. PubMed
CRISPR/Cas9-Mediated Deletion of Foxn1 in NOD/SCID/IL2rg(-/-) Mice Results in Severe Immunodeficiency. Wei X, Lai Y, Li B, Qin L, Xu Y, Lin S, Wang S, Wu Q, Liang Q, Huang G, Deng Q, Liu P, Wu D, Lai L, Yao Y, Li P. Sci Rep. 2017 Aug 10;7(1):7720. doi: 10.1038/s41598-017-08337-8. PubMed
Multiple homologous genes knockout (KO) by CRISPR/Cas9 system in rabbit. Liu H, Sui T, Liu D, Liu T, Chen M, Deng J, Xu Y, Li Z. Gene. 2018 Mar 20;647:261-267. doi: 10.1016/j.gene.2018.01.044. Epub 2018 Jan 12. PubMed
CRISPR/Cas9-mediated mosaic mutation of SRY gene induces hermaphroditism in rabbits. Song Y, Xu Y, Liang M, Zhang Y, Chen M, Deng J, Li Z. Biosci Rep. 2018 Mar 21;38(2). pii: BSR20171490. doi: 10.1042/BSR20171490. Print 2018 Apr 27. PubMed
Development of muscular dystrophy in a CRISPR-engineered mutant rabbit model with frame-disrupting ANO5 mutations. Sui T, Xu L, Lau YS, Liu D, Liu T, Gao Y, Lai L, Han R, Li Z. Cell Death Dis. 2018 May 22;9(6):609. doi: 10.1038/s41419-018-0674-y. PubMed
A novel rabbit model of Duchenne muscular dystrophy generated by CRISPR/Cas9. Sui T, Lau YS, Liu D, Liu T, Xu L, Gao Y, Lai L, Li Z, Han R. Dis Model Mech. 2018 Jun 4;11(6). pii: dmm.032201. doi: 10.1242/dmm.032201. PubMed
Generation and Phenotype Identification of PAX4 Gene Knockout Rabbit by CRISPR/Cas9 System. Xu Y, Wang Y, Song Y, Deng J, Chen M, Ouyang H, Lai L, Li Z. G3 (Bethesda). 2018 Jul 31;8(8):2833-2840. doi: 10.1534/g3.118.300448. PubMed
The disrupted balance between hair follicles and sebaceous glands in Hoxc13-ablated rabbits. Deng J, Chen M, Liu Z, Song Y, Sui T, Lai L, Li Z. FASEB J. 2019 Jan;33(1):1226-1234. doi: 10.1096/fj.201800928RR. Epub 2018 Aug 20. PubMed
LMNA-mutated Rabbits: A Model of Premature Aging Syndrome with Muscular Dystrophy and Dilated Cardiomyopathy. Sui T, Liu D, Liu T, Deng J, Chen M, Xu Y, Song Y, Ouyang H, Lai L, Li Z. Aging Dis. 2019 Feb 1;10(1):102-115. doi: 10.14336/AD.2018.0209. eCollection 2019 Feb. PubMed
Mutations of GADD45G in rabbits cause cleft lip by the disorder of proliferation, apoptosis and epithelial-mesenchymal transition (EMT). Lu Y, Liang M, Zhang Q, Liu Z, Song Y, Lai L, Li Z. Biochim Biophys Acta Mol Basis Dis. 2019 Sep 1;1865(9):2356-2367. doi: 10.1016/j.bbadis.2019.05.015. Epub 2019 May 29. PubMed
CRISPR/Cas9-mediated Disruption of Fibroblast Growth Factor 5 in Rabbits Results in a Systemic Long Hair Phenotype by Prolonging Anagen. Xu Y, Liu H, Pan H, Wang X, Zhang Y, Yao B, Li N, Lai L, Li Z. Genes (Basel). 2020 Mar 11;11(3). pii: genes11030297. doi: 10.3390/genes11030297. PubMed
CRISPR/Cas9-mediated beta-globin gene knockout in rabbits recapitulates human beta-thalassemia. Yang Y, Kang X, Hu S, Chen B, Xie Y, Song B, Zhang Q, Wu H, Ou Z, Xian Y, Fan Y, Li X, Lai L, Sun X. J Biol Chem. 2021 Jan-Jun;296:100464. doi: 10.1016/j.jbc.2021.100464. Epub 2021 Feb 25. PubMed
CRISPR/Cas9-Mediated Specific Integration of Fat-1 and IGF-1 at the pRosa26 Locus. You W, Li M, Qi Y, Wang Y, Chen Y, Liu Y, Li L, Ouyang H, Pang D. Genes (Basel). 2021 Jul 1;12(7). pii: genes12071027. doi: 10.3390/genes12071027. PubMed
A synonymous mutation in IGF-1 impacts the transcription and translation process of gene expression. Wang SY, Cheng YY, Liu SC, Xu YX, Gao Y, Wang CL, Wang ZG, Feng TQ, Lu GH, Song J, Xia PJ, Hao LL. Mol Ther Nucleic Acids. 2021 Aug 19;26:1446-1465. doi: 10.1016/j.omtn.2021.08.007. eCollection 2021 Dec 3. PubMed
CRISPR/Cas9-Mediated Models of Retinitis Pigmentosa Reveal Differential Proliferative Response of Muller Cells between Xenopus laevis and Xenopus tropicalis. Parain K, Lourdel S, Donval A, Chesneau A, Borday C, Bronchain O, Locker M, Perron M. Cells. 2022 Feb 25;11(5). pii: cells11050807. doi: 10.3390/cells11050807. PubMed
CRISPR/Cas9-mediated deletion of Fam83h induces defective tooth mineralization and hair development in rabbits. Zhang Y, Yang J, Yao H, Zhang Z, Song Y. J Cell Mol Med. 2022 Nov;26(22):5670-5679. doi: 10.1111/jcmm.17597. Epub 2022 Oct 27. PubMed
CRISPR-Cas9-mediated deletion of carbonic anhydrase 2 in the ciliary body to treat glaucoma. Jiang J, Kong K, Fang X, Wang D, Zhang Y, Wang P, Yang Z, Zhang Y, Liu X, Aung T, Li F, Yu-Wai-Man P, Zhang X. Cell Rep Med. 2024 May 21;5(5):101524. doi: 10.1016/j.xcrm.2024.101524. Epub 2024 Apr 25. PubMed

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