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Purpose(Empty Backbone) gRNA expression plasmid
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51306 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC57-Simple
- Backbone size (bp) 2710
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Vector typeCRISPR ; Xenopus expression
- Promoter T7
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13R
- 3′ sequencing primer M13F (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC57-Simple-gRNA backbone was a gift from Yonglong Chen (Addgene plasmid # 51306 ; http://n2t.net/addgene:51306 ; RRID:Addgene_51306) -
For your References section:
Efficient RNA/Cas9-mediated genome editing in Xenopus tropicalis. Guo X, Zhang T, Hu Z, Zhang Y, Shi Z, Wang Q, Cui Y, Wang F, Zhao H, Chen Y. Development. 2014 Jan 8. 10.1242/dev.099853 PubMed 24401372
