-
PurposeExpression of SOX4 after doxycycline administration
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 51304 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepINDUCER21 (ORF-EG)
-
Backbone manufacturerDr. Richard Mulligan
- Backbone size w/o insert (bp) 12152
- Total vector size (bp) 13577
-
Modifications to backboneNone
-
Vector typeLentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)Stbl3
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameSRY (sex determining region Y)-box 4
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1425
-
Mutationfull length ORF
-
GenBank IDNM_003107.2
-
Entrez GeneSOX4 (a.k.a. CSS10, EVI16)
-
Tag
/ Fusion Protein
- n/a
Resource Information
-
A portion of this plasmid was derived from a plasmid made bypINDUCER21 from Dr. Steve Elledge (HHMI) Full lenght ORF obtained as pDONR construct from Genecopoeia catalog #: Y2028
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pINDUCER21-SOX4 was a gift from George Daley (Addgene plasmid # 51304 ; http://n2t.net/addgene:51304 ; RRID:Addgene_51304) -
For your References section:
Induction of multipotential hematopoietic progenitors from human pluripotent stem cells via respecification of lineage-restricted precursors. Doulatov S, Vo LT, Chou SS, Kim PG, Arora N, Li H, Hadland BK, Bernstein ID, Collins JJ, Zon LI, Daley GQ. Cell Stem Cell. 2013 Oct 3;13(4):459-70. doi: 10.1016/j.stem.2013.09.002. 10.1016/j.stem.2013.09.002 PubMed 24094326