pINDUCER21-ERG
(Plasmid #51301)

• Purpose: Expression of ERG after doxycycline administration
• Depositing Lab: George Daley
• Publication: Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70. doi: 10.1016/j.stem.2013.09.002.

Backbone

• Vector backbone: pINDUCER21 (ORF-EG)
• Backbone manufacturer: Dr. Richard Mulligan
• Backbone size w/o insert (bp): 12152
• Total vector size (bp): 13592
• Modifications to backbone: None
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: v-ets avian erythroblastosis virus E26 oncogene homolog
• Species: H. sapiens (human)
• Insert Size (bp): 1440
• Mutation: full length ORF
• GenBank ID: NM_182918.3
• Entrez Gene: ERG (a.k.a. LMPHM14, erg-3, p55)
• Tag / Fusion Protein:
  • n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pINDUCER21 from Dr. Steve Elledge (HHMI) Full lenght ORF obtained as pDONR construct from Genecopoeia catalog #: Z1500

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

NOTE: The map and depositor sequence are of the empty backbone ONLY. Addgene does not have sequence for the fully assembled plasmid. Sequence for much of the insert can be found in Addgene's sequencing results.