pCIOX
(Plasmid #51300)

• Purpose: (Empty Backbone) E.coli expression vector for His8-SUMO tagged recombinant protein
• Depositing Lab: Andrea Mattevi
• Publication: pCIOX (unpublished)

Backbone

• Vector backbone: pET SUMO
• Backbone manufacturer: Invitrogen
• Backbone size (bp): 5643
• Modifications to backbone: 1) MultiCloning Site (MCS) of pET28b vector inserted in the pET SUMO cloning site 2) Addition of 2 codon for His residues to the 6 already present in the original vector 3) Removal of the original EcoRI site located inside SUMO ORF 4) Removal of the original HindIII site
• Vector type: Bacterial Expression
• Promoter: T7
• Tag / Fusion Protein:
  • His8 + SUMO (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 reverse

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This vector is a modified version of the commercial (sell as linearized) pET SUMO vector of Invitrogen. This vector has been optimized for restriction enzyme cloning, thanks to the inserted MultiCloning Site of pET28b.