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pcDNA3.1-mNRDc-E>A-V5
(Plasmid #51298)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51298 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NRDc E247A
  • Alt name
    Nrd1
  • Alt name
    nardilysin
  • Alt name
    N-arginine dibasic convertase
  • Species
    M. musculus (mouse)
  • Mutation
    E247A, enzymatic inactive mutant
  • GenBank ID
    230598
  • Entrez Gene
    Nrd1 (a.k.a. RP23-188A12.1, 2600011I06Rik, AI875733, NRD-C)
  • Promoter CMV
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • HIS (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGHrev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-mNRDc-E>A-V5 was a gift from Eiichiro Nishi (Addgene plasmid # 51298 ; http://n2t.net/addgene:51298 ; RRID:Addgene_51298)

  • For your References section:

    Critical roles of nardilysin in the maintenance of body temperature homoeostasis. Hiraoka Y, Matsuoka T, Ohno M, Nakamura K, Saijo S, Matsumura S, Nishi K, Sakamoto J, Chen PM, Inoue K, Fushiki T, Kita T, Kimura T, Nishi E. Nat Commun. 2014;5:3224. doi: 10.1038/ncomms4224. 10.1038/ncomms4224 PubMed 24492630
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