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Addgene

HBV 1.2-mer WT replicon
(Plasmid #51294)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51294 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGEM-4Z
  • Backbone manufacturer
    Promega
  • Total vector size (bp) 5971

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    1.2 units of the HBV genome
  • Species
    Hepatitis B virus
  • GenBank ID
    V01460 V01460

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer pQE-Fwd (5'-CCCGAAAAGTGCCACCTG-3')
  • 3′ sequencing primer M13-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note- Plasmid 51294: HBV 1.2-mer WT replicon should be handled under BSL-2 conditions. Vaccination is highly recommended for researchers working with this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HBV 1.2-mer WT replicon was a gift from Wang-Shick Ryu (Addgene plasmid # 51294)

  • For your References section:

    Stimulation of hepatitis B virus genome replication by HBx is linked to both nuclear and cytoplasmic HBx expression. Cha MY, Ryu DK, Jung HS, Chang HE, Ryu WS. J Gen Virol. 2009 Apr;90(Pt 4):978-86. doi: 10.1099/vir.0.009928-0. Epub 2009 Mar 4. 10.1099/vir.0.009928-0 PubMed 19264639
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