pBudORF2-CH
(Plasmid #51289)

• Purpose: expresses the codon optimized ORF2 of L1RP with his tag
• Depositing Lab: Astrid Roy-Engel
• Publication: Wagstaff et al PLoS One. 2011 May 10;6(5):e19672. doi: 10.1371/journal.pone.0019672.

Backbone

• Vector backbone: pBudCE4.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4595
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Bleocin (Zeocin), 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: L1 ORF2
• Alt name: L1RE1
• Alt name: Line1
• Species: H. sapiens (human)
• Entrez Gene: L1RE1 (a.k.a. L1.2, LRE1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Myc (C terminal on backbone)
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EBV-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was created using the codon optimized L1RP as a source for the ORF2 coding sequence.