L1-neo-TET
(Plasmid #51284)

• Purpose: expresses codon optimized human L1 retrotransposon driven by CMV promoter and tagged with the self splicing intron neo cassette
• Depositing Lab: Astrid Roy-Engel
• Publication: Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi: 10.1371/journal.pgen.1000458. Epub 2009 Apr 24.

Backbone

• Vector backbone: pBluescript
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: L1RE1
• Alt name: L1
• Alt name: Line1
• Species: H. sapiens (human)
• Entrez Gene: L1RE1 (a.k.a. L1.2, LRE1)
• Promoter: CMV
• Tag / Fusion Protein:
  • neoTET cassette with a tetrahymena self-splicing intron interrupting the neo gene (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7 or CMV-F
• 3′ sequencing primer: Sp6

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

L1 is tagged with the self splicing intron neo cassette such that G418 resistance will be obtained only if retrotransposition occurs.